Staining:Article Title: CD8 + T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging.
Article Snippet: .. 14.rat anti Mac2 (1:250) Biolegend cat 125402 15.chicken anti MBP (1:1000) thermo fisher scientific cat PA1-10008 16.chicken anti-Neurofilament heavy polypeptide(1:400) abcam cat ab4680 17. goat anti PDGFR alpha (1:100) R&D Systems cat AF1062 18. goat anti CD69 (1:100) R&D Systems cat AF2386 19. goat anti PD-1 (1:100) R&D Systems cat AF1021 20. rabbit anti-LAG-3 antibody (1:100) Abcam cat ab209238 21.CD16/CD32 Monoclonal Antibody (1:100) eBioscience cat 14-0161-82 22.CD11b (PE/Cy7, M1/70, 1:200) eBioscience Cat:48-0451-82 anti mouse 555 thermo fisher scientific (1:500) cat A-21422 anti mouse 647 thermo fisher scientific (1:500) cat A-21235 anti mouse 488 thermo fisher scientific (1:500) cat A-21202 anti rabbit 555 thermo fisher scientific ( 1:500) cat A-21428 anti rabbit 488 thermo fisher scientific (1:500) cat A-11008 anti rat 555 thermo fisher scientific (1:500) cat A-21434 anti goat 555 thermo fisher scientific (1:500) cat A-32116 anti rat 488 thermo fisher scientific (1:500) cat A-11006 anti chicken 488 thermo fisher scientific (1:500) cat A-11039 anti chicken 555 thermo fisher scientific (1:500)cat A-32932 Streptavidin, Alexa Fluor TM 555 conjugate thermo fisher scientific (1:500) cat S32355 AF1062FM green fluorescent myelin stain (thermo fisher scientific F34651,1:400) Donkey anti-Rat 488 thermo fisher scientific (1:500) cat A-21432 Donkey anti-Goat 555 thermo fisher scientific (1:500) cat A32816 Donkey anti-Rabbit 647 thermo fisher scientific (1:500) cat A-31573 Rat anti PD-1 bxcell cat BE0146 Rat isotype control bxcell cat BE0089 Hamster anti CTLA-4 bxcell cat BP0131 Hamster isotype control bxcell cat BP0087 Validation All the primary antibodies were validated by extensive previous studies and by the manufacturers. .. !%2Bthermo%20%2Banti%20% 2Brabbit&cid=bid_pca_aus_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_pur_con https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-11008 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21434 https://www.thermofisher.com/antibody/product/Rabbit-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21431 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006 https://www.thermofisher.com/order/catalog/product/S21381#/S21381 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A32932 https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21432 https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21208 https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-31573 in vivo treatment https://bxcell.com/product/invivomab-anti-m-pd-1/ https://bxcell.com/product/rat-igg2a-isotype-control/ https://bxcell.com/product/m-cd152-m-ctla-4-2/ https://bxcell.com/product/invivoplus-polyclonal-syrian-hamster-igg/ Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Mouse: C57BL/6J Janvier Labs, CSD animal facility, University Hospital animal facility.
Article Title: CD8 + T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
Article Snippet: .. 14.rat anti Mac2 (1:250) Biolegend cat 125402 15.chicken anti MBP (1:1000) thermo fisher scientific cat PA1-10008 16.chicken anti-Neurofilament heavy polypeptide(1:400) abcam cat ab4680 17. goat anti PDGFR alpha (1:100) R&D Systems cat AF1062 18. goat anti CD69 (1:100) R&D Systems cat AF2386 19. goat anti PD-1 (1:100) R&D Systems cat AF1021 20. rabbit anti-LAG-3 antibody (1:100) Abcam cat ab209238 21.CD16/CD32 Monoclonal Antibody (1:100) eBioscience cat 14-0161-82 22.CD11b (PE/Cy7, M1/70, 1:200) eBioscience Cat:48-0451-82 anti mouse 555 thermo fisher scientific (1:500) cat A-21422 anti mouse 647 thermo fisher scientific (1:500) cat A-21235 anti mouse 488 thermo fisher scientific (1:500) cat A-21202 anti rabbit 555 thermo fisher scientific ( 1:500) cat A-21428 anti rabbit 488 thermo fisher scientific (1:500) cat A-11008 anti rat 555 thermo fisher scientific (1:500) cat A-21434 anti goat 555 thermo fisher scientific (1:500) cat A-32116 anti rat 488 thermo fisher scientific (1:500) cat A-11006 anti chicken 488 thermo fisher scientific (1:500) cat A-11039 anti chicken 555 thermo fisher scientific (1:500)cat A-32932 Streptavidin, Alexa Fluor TM 555 conjugate thermo fisher scientific (1:500) cat S32355 AF1062FM green fluorescent myelin stain (thermo fisher scientific F34651,1:400) Donkey anti-Rat 488 thermo fisher scientific (1:500) cat A-21432 Donkey anti-Goat 555 thermo fisher scientific (1:500) cat A32816 Donkey anti-Rabbit 647 thermo fisher scientific (1:500) cat A-31573 Rat anti PD-1 bxcell cat BE0146 Rat isotype control bxcell cat BE0089 Hamster anti CTLA-4 bxcell cat BP0131 Hamster isotype control bxcell cat BP0087 Validation All the primary antibodies were validated by extensive previous studies and by the manufacturers. .. !%2Bthermo%20%2Banti%20% 2Brabbit&cid=bid_pca_aus_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_pur_con https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-11008 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21434 https://www.thermofisher.com/antibody/product/Rabbit-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21431 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006 https://www.thermofisher.com/order/catalog/product/S21381#/S21381 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A32932 https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21432 https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21208 https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-31573 in vivo treatment https://bxcell.com/product/invivomab-anti-m-pd-1/ https://bxcell.com/product/rat-igg2a-isotype-control/ https://bxcell.com/product/m-cd152-m-ctla-4-2/ https://bxcell.com/product/invivoplus-polyclonal-syrian-hamster-igg/ Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Mouse: C57BL/6J Janvier Labs, CSD animal facility, University Hospital animal facility.
Control:Article Title: CD8 + T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging.
Article Snippet: .. 14.rat anti Mac2 (1:250) Biolegend cat 125402 15.chicken anti MBP (1:1000) thermo fisher scientific cat PA1-10008 16.chicken anti-Neurofilament heavy polypeptide(1:400) abcam cat ab4680 17. goat anti PDGFR alpha (1:100) R&D Systems cat AF1062 18. goat anti CD69 (1:100) R&D Systems cat AF2386 19. goat anti PD-1 (1:100) R&D Systems cat AF1021 20. rabbit anti-LAG-3 antibody (1:100) Abcam cat ab209238 21.CD16/CD32 Monoclonal Antibody (1:100) eBioscience cat 14-0161-82 22.CD11b (PE/Cy7, M1/70, 1:200) eBioscience Cat:48-0451-82 anti mouse 555 thermo fisher scientific (1:500) cat A-21422 anti mouse 647 thermo fisher scientific (1:500) cat A-21235 anti mouse 488 thermo fisher scientific (1:500) cat A-21202 anti rabbit 555 thermo fisher scientific ( 1:500) cat A-21428 anti rabbit 488 thermo fisher scientific (1:500) cat A-11008 anti rat 555 thermo fisher scientific (1:500) cat A-21434 anti goat 555 thermo fisher scientific (1:500) cat A-32116 anti rat 488 thermo fisher scientific (1:500) cat A-11006 anti chicken 488 thermo fisher scientific (1:500) cat A-11039 anti chicken 555 thermo fisher scientific (1:500)cat A-32932 Streptavidin, Alexa Fluor TM 555 conjugate thermo fisher scientific (1:500) cat S32355 AF1062FM green fluorescent myelin stain (thermo fisher scientific F34651,1:400) Donkey anti-Rat 488 thermo fisher scientific (1:500) cat A-21432 Donkey anti-Goat 555 thermo fisher scientific (1:500) cat A32816 Donkey anti-Rabbit 647 thermo fisher scientific (1:500) cat A-31573 Rat anti PD-1 bxcell cat BE0146 Rat isotype control bxcell cat BE0089 Hamster anti CTLA-4 bxcell cat BP0131 Hamster isotype control bxcell cat BP0087 Validation All the primary antibodies were validated by extensive previous studies and by the manufacturers. .. !%2Bthermo%20%2Banti%20% 2Brabbit&cid=bid_pca_aus_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_pur_con https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-11008 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21434 https://www.thermofisher.com/antibody/product/Rabbit-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21431 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006 https://www.thermofisher.com/order/catalog/product/S21381#/S21381 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A32932 https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21432 https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21208 https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-31573 in vivo treatment https://bxcell.com/product/invivomab-anti-m-pd-1/ https://bxcell.com/product/rat-igg2a-isotype-control/ https://bxcell.com/product/m-cd152-m-ctla-4-2/ https://bxcell.com/product/invivoplus-polyclonal-syrian-hamster-igg/ Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Mouse: C57BL/6J Janvier Labs, CSD animal facility, University Hospital animal facility.
Article Title: CD8 + T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
Article Snippet: .. 14.rat anti Mac2 (1:250) Biolegend cat 125402 15.chicken anti MBP (1:1000) thermo fisher scientific cat PA1-10008 16.chicken anti-Neurofilament heavy polypeptide(1:400) abcam cat ab4680 17. goat anti PDGFR alpha (1:100) R&D Systems cat AF1062 18. goat anti CD69 (1:100) R&D Systems cat AF2386 19. goat anti PD-1 (1:100) R&D Systems cat AF1021 20. rabbit anti-LAG-3 antibody (1:100) Abcam cat ab209238 21.CD16/CD32 Monoclonal Antibody (1:100) eBioscience cat 14-0161-82 22.CD11b (PE/Cy7, M1/70, 1:200) eBioscience Cat:48-0451-82 anti mouse 555 thermo fisher scientific (1:500) cat A-21422 anti mouse 647 thermo fisher scientific (1:500) cat A-21235 anti mouse 488 thermo fisher scientific (1:500) cat A-21202 anti rabbit 555 thermo fisher scientific ( 1:500) cat A-21428 anti rabbit 488 thermo fisher scientific (1:500) cat A-11008 anti rat 555 thermo fisher scientific (1:500) cat A-21434 anti goat 555 thermo fisher scientific (1:500) cat A-32116 anti rat 488 thermo fisher scientific (1:500) cat A-11006 anti chicken 488 thermo fisher scientific (1:500) cat A-11039 anti chicken 555 thermo fisher scientific (1:500)cat A-32932 Streptavidin, Alexa Fluor TM 555 conjugate thermo fisher scientific (1:500) cat S32355 AF1062FM green fluorescent myelin stain (thermo fisher scientific F34651,1:400) Donkey anti-Rat 488 thermo fisher scientific (1:500) cat A-21432 Donkey anti-Goat 555 thermo fisher scientific (1:500) cat A32816 Donkey anti-Rabbit 647 thermo fisher scientific (1:500) cat A-31573 Rat anti PD-1 bxcell cat BE0146 Rat isotype control bxcell cat BE0089 Hamster anti CTLA-4 bxcell cat BP0131 Hamster isotype control bxcell cat BP0087 Validation All the primary antibodies were validated by extensive previous studies and by the manufacturers. .. !%2Bthermo%20%2Banti%20% 2Brabbit&cid=bid_pca_aus_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_pur_con https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-11008 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21434 https://www.thermofisher.com/antibody/product/Rabbit-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21431 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006 https://www.thermofisher.com/order/catalog/product/S21381#/S21381 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A32932 https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21432 https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21208 https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-31573 in vivo treatment https://bxcell.com/product/invivomab-anti-m-pd-1/ https://bxcell.com/product/rat-igg2a-isotype-control/ https://bxcell.com/product/m-cd152-m-ctla-4-2/ https://bxcell.com/product/invivoplus-polyclonal-syrian-hamster-igg/ Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Mouse: C57BL/6J Janvier Labs, CSD animal facility, University Hospital animal facility.
Biomarker Discovery:Article Title: CD8 + T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging.
Article Snippet: .. 14.rat anti Mac2 (1:250) Biolegend cat 125402 15.chicken anti MBP (1:1000) thermo fisher scientific cat PA1-10008 16.chicken anti-Neurofilament heavy polypeptide(1:400) abcam cat ab4680 17. goat anti PDGFR alpha (1:100) R&D Systems cat AF1062 18. goat anti CD69 (1:100) R&D Systems cat AF2386 19. goat anti PD-1 (1:100) R&D Systems cat AF1021 20. rabbit anti-LAG-3 antibody (1:100) Abcam cat ab209238 21.CD16/CD32 Monoclonal Antibody (1:100) eBioscience cat 14-0161-82 22.CD11b (PE/Cy7, M1/70, 1:200) eBioscience Cat:48-0451-82 anti mouse 555 thermo fisher scientific (1:500) cat A-21422 anti mouse 647 thermo fisher scientific (1:500) cat A-21235 anti mouse 488 thermo fisher scientific (1:500) cat A-21202 anti rabbit 555 thermo fisher scientific ( 1:500) cat A-21428 anti rabbit 488 thermo fisher scientific (1:500) cat A-11008 anti rat 555 thermo fisher scientific (1:500) cat A-21434 anti goat 555 thermo fisher scientific (1:500) cat A-32116 anti rat 488 thermo fisher scientific (1:500) cat A-11006 anti chicken 488 thermo fisher scientific (1:500) cat A-11039 anti chicken 555 thermo fisher scientific (1:500)cat A-32932 Streptavidin, Alexa Fluor TM 555 conjugate thermo fisher scientific (1:500) cat S32355 AF1062FM green fluorescent myelin stain (thermo fisher scientific F34651,1:400) Donkey anti-Rat 488 thermo fisher scientific (1:500) cat A-21432 Donkey anti-Goat 555 thermo fisher scientific (1:500) cat A32816 Donkey anti-Rabbit 647 thermo fisher scientific (1:500) cat A-31573 Rat anti PD-1 bxcell cat BE0146 Rat isotype control bxcell cat BE0089 Hamster anti CTLA-4 bxcell cat BP0131 Hamster isotype control bxcell cat BP0087 Validation All the primary antibodies were validated by extensive previous studies and by the manufacturers. .. !%2Bthermo%20%2Banti%20% 2Brabbit&cid=bid_pca_aus_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_pur_con https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-11008 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21434 https://www.thermofisher.com/antibody/product/Rabbit-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21431 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006 https://www.thermofisher.com/order/catalog/product/S21381#/S21381 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A32932 https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21432 https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21208 https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-31573 in vivo treatment https://bxcell.com/product/invivomab-anti-m-pd-1/ https://bxcell.com/product/rat-igg2a-isotype-control/ https://bxcell.com/product/m-cd152-m-ctla-4-2/ https://bxcell.com/product/invivoplus-polyclonal-syrian-hamster-igg/ Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Mouse: C57BL/6J Janvier Labs, CSD animal facility, University Hospital animal facility.
Article Title: CD8 + T cells induce interferon-responsive oligodendrocytes and microglia in white matter aging
Article Snippet: .. 14.rat anti Mac2 (1:250) Biolegend cat 125402 15.chicken anti MBP (1:1000) thermo fisher scientific cat PA1-10008 16.chicken anti-Neurofilament heavy polypeptide(1:400) abcam cat ab4680 17. goat anti PDGFR alpha (1:100) R&D Systems cat AF1062 18. goat anti CD69 (1:100) R&D Systems cat AF2386 19. goat anti PD-1 (1:100) R&D Systems cat AF1021 20. rabbit anti-LAG-3 antibody (1:100) Abcam cat ab209238 21.CD16/CD32 Monoclonal Antibody (1:100) eBioscience cat 14-0161-82 22.CD11b (PE/Cy7, M1/70, 1:200) eBioscience Cat:48-0451-82 anti mouse 555 thermo fisher scientific (1:500) cat A-21422 anti mouse 647 thermo fisher scientific (1:500) cat A-21235 anti mouse 488 thermo fisher scientific (1:500) cat A-21202 anti rabbit 555 thermo fisher scientific ( 1:500) cat A-21428 anti rabbit 488 thermo fisher scientific (1:500) cat A-11008 anti rat 555 thermo fisher scientific (1:500) cat A-21434 anti goat 555 thermo fisher scientific (1:500) cat A-32116 anti rat 488 thermo fisher scientific (1:500) cat A-11006 anti chicken 488 thermo fisher scientific (1:500) cat A-11039 anti chicken 555 thermo fisher scientific (1:500)cat A-32932 Streptavidin, Alexa Fluor TM 555 conjugate thermo fisher scientific (1:500) cat S32355 AF1062FM green fluorescent myelin stain (thermo fisher scientific F34651,1:400) Donkey anti-Rat 488 thermo fisher scientific (1:500) cat A-21432 Donkey anti-Goat 555 thermo fisher scientific (1:500) cat A32816 Donkey anti-Rabbit 647 thermo fisher scientific (1:500) cat A-31573 Rat anti PD-1 bxcell cat BE0146 Rat isotype control bxcell cat BE0089 Hamster anti CTLA-4 bxcell cat BP0131 Hamster isotype control bxcell cat BP0087 Validation All the primary antibodies were validated by extensive previous studies and by the manufacturers. .. !%2Bthermo%20%2Banti%20% 2Brabbit&cid=bid_pca_aus_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_pur_con https://www.thermofisher.com/antibody/product/Goat-anti-Rabbit-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-11008 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21434 https://www.thermofisher.com/antibody/product/Rabbit-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21431 https://www.thermofisher.com/antibody/product/Goat-anti-Rat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-11006 https://www.thermofisher.com/order/catalog/product/S21381#/S21381 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Secondary-Antibody-Polyclonal/A-11039 https://www.thermofisher.com/antibody/product/Goat-anti-Chicken-IgY-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A32932 https://www.thermofisher.com/antibody/product/Donkey-anti-Goat-IgG-H-L-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21432 https://www.thermofisher.com/antibody/product/Donkey-anti-Rat-IgG-H-L-Highly-Cross-Adsorbed-Secondary-Antibody-Polyclonal/ A-21208 https://www.thermofisher.com/antibody/product/Donkey-anti-Rabbit-IgG-H-L-Highly-Cross-Adsorbed-Secondary-AntibodyPolyclonal/A-31573 in vivo treatment https://bxcell.com/product/invivomab-anti-m-pd-1/ https://bxcell.com/product/rat-igg2a-isotype-control/ https://bxcell.com/product/m-cd152-m-ctla-4-2/ https://bxcell.com/product/invivoplus-polyclonal-syrian-hamster-igg/ Animals and other organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research Laboratory animals Mouse: C57BL/6J Janvier Labs, CSD animal facility, University Hospital animal facility.
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